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MAB1302

Sigma-Aldrich

Mouse Anti-Human IgG Antibody, clone HP6017, Fc

clone HP6017, Chemicon®, from mouse

Synonym(s):

Anti-CD32, Anti-CD32B, Anti-FCG2, Anti-FCGR2, Anti-FCGR2C, Anti-FcGRIIB, Anti-FcRII-c, Anti-FcgammaRIIb, Anti-IGFR2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

HP6017, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NM_001002273.1
NM_001002274.1
NM_001002275.1
NM_004001.3

UniProt accession no.

P31994

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FCGR2B(2213)

Specificity

Human IgG Fc fragment. ELISA Specificity: (Hamilton et al., 1987)

human IgG subclass 1 100%

human IgG subclass 2 100%

human IgG subclass 3 100%

human IgG subclass 4 100%

human IgG Pan 100%

human IgG Fc 100%

human IgG Fab .12%



Isoelectric Focusing: mean pI 8.0

Immunogen

Epitope: Fc fragment

Application

Detect Human IgG using this Mouse anti-Human IgG Antibody, clone HP6017, Fc validated for use in ELISA, RIA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Useful as both a capture antibody and a detection antibody for human IgG Fc (Papadea et al., 1985; Hamilton, et al., 1987).

ELISA

Radio-immunoassay (RIA)

Immunobloting.

Optimal working dilutions must be determined by end user.

Linkage

Replaces: CBL102

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02M PB, 0.25M NaCl with 0.1% sodium azide, pH=7.6.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gunnveig Grodeland et al.
Journal of immunology (Baltimore, Md. : 1950), 197(9), 3575-3585 (2016-09-28)
It has been difficult to translate promising results from DNA vaccination in mice to larger animals and humans. Previously, DNA vaccines encoding proteins that target Ag to MHC class II (MHC-II) molecules on APCs have been shown to induce rapid
Miguel Ángel Muñoz-Alía et al.
PLoS pathogens, 17(2), e1009283-e1009283 (2021-02-04)
The frequent overexpression of CD46 in malignant tumors has provided a basis to use vaccine-lineage measles virus (MeV) as an oncolytic virotherapy platform. However, widespread measles seropositivity limits the systemic deployment of oncolytic MeV for the treatment of metastatic neoplasia.
Kartik A Shah et al.
Biotechnology and bioengineering, 112(12), 2624-2629 (2015-06-03)
Monoclonal antibodies (mAbs) that bind and neutralize human pathogens have great therapeutic potential. Advances in automated screening and liquid handling have resulted in the ability to discover antigen-specific antibodies either directly from human blood or from various combinatorial libraries (phage

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