ABC459

Anti-NOX4 Antibody

from rabbit

• Synonym(s): NADPH oxidase 4, Kidney oxidase-1, KOX-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable
• NCBI accession no.: NP_001137308
• UniProt accession no.: Q9NPH5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NOX4(50507)

Description

General description

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex.

Immunogen

Linear peptide corresponding to the C-terminus of human NOX4.

Application

Anti-NOX4 Antibody is a highly specific rabbit polyclonal antibody, that targets NADPH Oxidase (phox) & has been tested in IHC & ICC.

Immunohistochemistry Analysis: A 1:500 and a 1:50 dilution from a representative lot detected NOX4 in human kidney and skeletal muscle tissues, respectively.

Immunohistochemistry Analysis:: A representative lot detected NOX4 in mouse liver tissue (I. Fabregat, IDIBELL, Barcelona, Spain).

Immunocytochemistry Analysis: A representative lot detected NOX4 in Hep3B cells (Esther Bertran, IDIBELL, Barcelona, Spain).

Immunohistochemistry Analysis: A representative lot detected NOX4 in mouse liver tissues expressing wildtype Mdr2/p19, or knockout Mdr2 ARF or knockout Stat3/Mdr2 (Sancho, P. et al. (2012). PLoS One. 7(9):e45285.).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected NOX4 in human kidney tissue.

Target description

67 kDa calculated

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable