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Key Documents

AB1728

Sigma-Aldrich

Anti-Connexin 43 Antibody, CT, cytosolic

Chemicon®, from rabbit

Synonym(s):

Gap Junction alpha-1 Protein (CxA-1)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... GJA1(2697)

General description

Connexin 43 (GJA1) is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5. Mouse Connexin 43 is a 382 amino acid gap junction protein with a predicted M.W. of ~43 kDa. It is prominently expressed in heart (Kumar, N. and Giula, N., 1996, Cell 84: 381-388).
Mouse Connexin 43 is a 382 amino acid gap junction protein with a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).

Specificity

Mouse C×43 immunogenic peptide sequence is specific for C×43 and no significant homology is seen with other connexins. The mouse C×43 peptide sequence is 100% homologous with rat and human C×43 (Beyer, E. et al., 1985, JCB 105: 2621).
Recognizes mouse Connexin 43.

Immunogen

Anti-Connexin 43 is made against a 23 amino acid C-terminal peptide sequence within the cytoplasmic domain of mouse C×43 (Beyer, E. and Steinberg, T. ,1991, JBC 266: 7971).
Epitope: C-term/cytosolic

Application

Anti-Connexin 43 Antibody, C-terminus, cytosolic is an antibody against Connexin 43 for use in ELISA, IF, IH, IP & WB.
Immunoprecipitation:
2-10 µg of a previous lot was used in immunoprecipitation.

ELISA:
A previous lot of this antibody was used at 1:10,000-100,000 dilution using 50 - 100 ng Cx43 control peptide (Catalog number AG636) per well.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Quality

Routinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected CONNEXIN 43 on 10 μg of Huvec lysates.

Target description

43 kDa

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, with 0.1% sodium azide as a preservative.

Storage and Stability

Stable for up to 1 year at 2-8°C in undiluted aliquots from date of receipt.

Analysis Note

Control
Positive Control: Heart tissue, mouse brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dakshesh Patel et al.
FEBS letters, 588(8), 1372-1378 (2014-01-18)
Connexin hemichannels are postulated to form a cell permeabilization pore for the uptake of fluorescent dyes and release of cellular ATP. Connexin hemichannel activity is enhanced by low external [Ca(2+)]o, membrane depolarization, metabolic inhibition, and some disease-causing gain-of-function connexin mutations.
Labeling human embryonic stem cell-derived cardiomyocytes with indocyanine green for noninvasive tracking with optical imaging: an FDA-compatible alternative to firefly luciferase.
Boddington, SE; Henning, TD; Jha, P; Schlieve, CR; Mandrussow, L; DeNardo, D; Bernstein et al.
Cell Transplantation null
Adi D Dubash et al.
The Journal of cell biology, 212(4), 425-438 (2016-02-10)
Members of the desmosome protein family are integral components of the cardiac area composita, a mixed junctional complex responsible for electromechanical coupling between cardiomyocytes. In this study, we provide evidence that loss of the desmosomal armadillo protein Plakophilin-2 (PKP2) in
Spontaneous cardiac calcinosis in BALB/cByJ mice.
Glass, AM; Coombs, W; Taffet, SM
Comparative Medicine null
Delayed wound closure and phenotypic changes in corneal epithelium of the spontaneously diabetic Goto-Kakizaki rat.
Wakuta, M; Morishige, N; Chikama, T; Seki, K; Nagano, T; Nishida, T
Investigative Ophthalmology & Visual Science null

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