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70922

BugBuster® HT Protein Extraction Reagent

Synonym(s):

Cell lysis reagent, Protein extraction buffer

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Size/SKUAvailabilityPrice
100 mL

Estimated to ship TODAY

$220.00
500 mL

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$859.00
1 L

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$1,490.00

About This Item

NACRES:
NA.77
UNSPSC Code:
41106511

$220.00


Estimated to ship TODAYDetails


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form

liquid

Quality Segment

manufacturer/tradename

Novagen®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

2-8°C

General description

BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Application

BugBuster® HT Protein Extraction Reagent has been used:
  • to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
  • for protein extraction from bacterial cell pellet
  • used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein

Features and Benefits

  • Fast protein extraction and nucleic acid degradation
  • Ideal for processing various samples of different volumes
  • Increased extraction is ensured by the addition of rLysozyme solution

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
70584-M7092171186
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

form

liquid

form

liquid

form

liquid

form

liquid

shipped in

wet ice

shipped in

-

shipped in

ambient

shipped in

wet ice

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

10-30°C

storage temp.

-

storage condition

OK to freeze

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze


Storage Class

10 - Combustible liquids

wgk

WGK 2



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