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Anti-Synapsin I Rabbit pAb

Name: Anti-Synapsin I Rabbit pAb
Brand: MM

lyophilized, Calbiochem^®

Synonym(s):

Rabbit Anti-Synapsin I, Synapsin I Detection Antibody

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

human, rat, mouse, bovine

Application:

—

Citations:

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Properties

biological source

rabbit

Quality Segment

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

does not contain

preservative

species reactivity

human, rat, mouse, bovine

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(ELISA (1:2500-1:10,000)
Frozen Sections
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate))

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Syn1(20964)

Description

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.

Recognizes the synapsin I protein in rat brain.

This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.

Immunogen

Bovine

purified, brovine brain synapsin I

Application

ELISA (1:2500-1:10,000)

Frozen Sections (see application references)

Immunoblotting (1:200)

Immunocytochemistry (1:500-1:2500)

Immunoprecipitation (1 µg/200 µg lysate)

Physical form

Lyophilized from 5 mM ammonium bicarbonate.

Preparation Note

Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Rat brain

Other Notes

Stone, L.M., et al. 1994. J. Neurosci.14, 301.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.

This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item	S8067	574778	AB1543P
Sigma-Aldrich 574777 Anti-Synapsin I Rabbit pAb	Sigma-Aldrich S8067 Anti-phospho-Synapsin I (pSer⁹) antibody produced in rabbit	Sigma-Aldrich 574778 Anti-Synapsin I Rabbit pAb	Sigma-Aldrich AB1543P Anti-Synapsin I Antibody
description lyophilized, Calbiochem^®	description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description -	description Chemicon^®, from rabbit
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form -	antibody form affinity purified immunoglobulin
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 100
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
shipped in ambient	shipped in dry ice	shipped in -	shipped in dry ice
Gene Information mouse ... Syn1(20964)	Gene Information human ... SYN1(6853) mouse ... Syn1(20964) rat ... Syn1(24949)	Gene Information -	Gene Information human ... SYN1(6853)
species reactivity human, rat, mouse, bovine	species reactivity Xenopus, human, chicken, rat, mouse	species reactivity (bovine, human, mouse, rat)	species reactivity human, mouse, rat, bovine

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Storage Class

11 - Combustible Solids

WGK

WGK 1

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