444232

MMP-9, Dimer, Human Neutrophil

• Synonym(s): Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase
• Enzyme Commission number: 3.4.24.35 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Assay: ≥90% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• specific activity: ≥1000 mU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• foreign activity: other MMP activity, none detected
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.

M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.

Physical form

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Preparation Note

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Olson, M.W. et al. 2000. J. Biol. Chem.275, 2661.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable