208902

Calpastatin Peptide

A 27-amino acid, cell-permeable peptide encoded by exon 1B of human calpastatin that acts as a potent inhibitor of calpain I and calpain II (IC₅₀ = 20 nM for purified rabbit calpain II).

• Synonym(s): Calpastatin Peptide, CS Peptide
• Empirical Formula (Hill Notation): C₁₄₂H₂₃₀N₃₆O₄₄S
• Molecular Weight: 3177.63
• NACRES: NA.77
• UNSPSC Code: 12352202
• Assay: ≥95% (HPLC)
• Form: lyophilized solid
• Storage condition: OK to freeze, desiccated (hygroscopic)

Properties

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• color: white
• solubility: 1% acetic acid: 1 mg/mL, water: 1 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A 27-amino acid, cell-permeable peptide encoded by exon 1B of human calpastatin that acts as a potent inhibitor of calpain I and calpain II (IC₅₀ = 20 nM for purified rabbit calpain II). Does not inhibit either papain or trypsin. Blocks the down-regulation of protein kinase C_ε (PKC_ε) in rat pituitary GH₄C₁ cells stimulated by thyrotropin-releasing hormone (TRH).

A 27-amino acid, peptide encoded by exon 1B of human calpastatin that acts as a cell-permeable and potent inhibitor of calpain I and calpain II (IC₅₀ = 20 nM for purified rabbit calpain II). Does not inhibit either papain or trypsin. Blocks the down-regulation of protein kinase C_ε (PKC_ε) in rat pituitary GH4C1 cells stimulated by thyrotropin-releasing hormone (TRH).

Biochem/physiol Actions

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 20 nM against calpain II

Cell permeable: yes

Primary Target
calpain 2

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Ac-Asp-Pro-Met-Ser-Ser-Thr-Tyr-Ile-Glu-Glu-Leu-Gly-Lys-Arg-Glu-Val-Thr-Ile-Pro-Pro-Lys-Tyr-Arg-Glu-Leu-Leu-Ala-NH₂

Eto, A., et al. 1995. J. Biol. Chem. 270, 25115.
Kawasaki, J., et al. 1989. J. Biochem. 106, 274.
Maki, M., et al. 1989. J. Biol. Chem. 264, 18866.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable