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LiChrospher® 100 DIOL (5 µm) HPLC Columns

HPLC sorbent

Synonym(s):

TM=["LiChrospher"] Diol sorbent for HPLC

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

LiChrospher® 100 DIOL (5 µm),

material

stainless steel column

Agency

suitable for USP L20

product line

LiChrospher®

feature

endcapped: no

extent of labeling

8% Carbon loading

parameter

60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)

surface area

350 m2/g

matrix

fully porous particle

matrix active group

Diol bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH range

2-7.5

bulk density

200‑400 kg/m3

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

storage temp.

no temp limit

General description

LiChrospher® DIOL has polar and hydrophobic properties, thus can be used as less polar alternative to LiChrospher Si 60 in normal phase applications or as less hydrophobic alternative to LiChrospher RP-8 in reversed phase applications. LiChrospher DIOL is also suitable for exclusion chromatography.

Application


  • Chiral and achiral separation of ten flavanones using supercritical fluid chromatography: This study illustrates the application of LiChrospher 100 DIOL in separating flavanones in bee pollen, utilizing both chiral and achiral approaches. The method offers enhanced accuracy and efficiency for analyzing natural products (Ares et al., 2022).

  • Application of Diol column under adsorption and mixed partition-adsorption normal-phase liquid chromatography mode: This research demonstrates the use of LiChrospher 100 DIOL for the separation of purines and pyrimidines, showcasing its versatility in both adsorption and mixed partition-adsorption chromatographic techniques, which are critical for biochemical analysis (Kazoka, 2008).

Analysis Note

Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
stability testing: passes test
Particle Size (d10): 4.8 - 5.5 µm
Particle Size (d50): 6.5 - 7.5 µm
Particle Size (d90): 9.0 - 10.5 µm

Legal Information

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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