Rapid determination of vitamin D₃ in milk-based infant formulas by liquid chromatography-tandem mass spectrometry.

Rapid determination of vitamin D₃ in milk-based infant formulas by liquid chromatography-tandem mass spectrometry.

Food chemistry (2014-07-21)

Byung-Man Kwak, In-Seek Jeong, Moon-Seok Lee, Jang-Hyuk Ahn, Jong-Su Park

RESUMEN

A rapid and simple sample preparation method for vitamin D3 (cholecalciferol) was developed for emulsified dairy products such as milk-based infant formulas. A sample was mixed in a 50 mL centrifuge tube with the same amount of water and isopropyl alcohol to achieve chemical extraction. Ammonium sulfate was used to induce phase separation. No-heating saponification was performed in the sample tube by adding KOH, NaCl, and NH3. Vitamin D3 was then separated and quantified using liquid chromatography-tandem mass spectrometry. The results for added recovery tests were in the range 93.11-110.65%, with relative standard deviations between 2.66% and 2.93%. The results, compared to those obtained using a certified reference material (SRM 1849a), were within the range of the certificated values. This method could be implemented in many laboratories that require time and labour saving.

MATERIALES

Referencia del producto

Marca

Descripción del producto

34863

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