808647

TSKgel^® Chelate-5PW (20 µm) HPLC Columns

L x I.D. 2.5 cm × 6 mm, iminodiacetic acid phase

• Sinónimos: TSKgel^® Chelate-5PW HPLC Column and Guardgel Kit
• UNSPSC Code: 41115700

Propiedades

• product name: TSKgel^® Chelate-5PW Guardgel Kit, phase iminodiacetic acid, L × I.D. 2.5 cm × 6 mm, 20 μm particle size
• material: PEEK
• product line: TSKgel^®
• manufacturer/tradename: Tosoh Chelate-5PW, 08647
• extent of labeling: 20 μmol/mL resin ligand density
• parameter: ≤20% organic solvent; ≤3 M salt concentration; ≤45 °C temperature
• technique(s): LPLC: suitable
• L × I.D.: 2.5 cm × 6 mm
• matrix: polymer particle platform
• matrix active group: iminodiacetic acid phase
• particle size: 20 μm
• operating pH: 2-12
• compatibility: mode of use affinity HPLC
• separation technique: affinity

Descripción

General description

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

Components

TSKgel Guardgel kits contain:

• 5 mL Guardgel packing material
• Column holder, consisting of external reducing union, cap, frit, 2 ferrules
• Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"
• 2 Replacement frits
• Capillary tubing, 5 cm x 1/16" OD x 0.01"
• Nuts and ferrules, 10-32 threads for 1/16" tubing, 2 each
• All hardware is 316 stainless steel

Dimensions for Guardgel kits for 21.5 mm ID columns:

• Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
• Capillary tubing, 5 cm x 1/16" OD x 0.02" ID

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation