V7514

Anti-VAMP8 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-endobrevin, Anti-vesicle-associated membrane protein 8
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~14 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): western blot: 1-2 μg/mL using HEK-293T cells expressing human VAMP8.
• UniProt accession no.: Q9BV40
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VAMP8(8673) ; mouse ... Vamp8(22320) ; rat ... Vamp8(83730)

Descripción

General description

Vesicle-associated membrane protein 8 (VAMP-8/endobrevin) is located at 2p11.2 on the human chromosome.

Immunogen

synthetic peptide corresponding to amino acids 38-56 of human VAMP8, conjugated to KLH. This sequence is highly conserved in mouse and rat VAMP8 (single amino acid substitution).

Application

Anti-VAMP-8 antibody has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Vesicle-associated membrane protein 8 (VAMP-8/endobrevin) is as an endosomal v-SNARE (vesicles or granules-soluble NSF attachment protein receptors) involved in fusion between early and late endosomes. It can interact with syntaxin-7, syntaxin-8, and Vti1b (Vesicle transport through interaction with t-SNAREs homolog 1B) to form an endosomal fusion complex. VAMP8 interacts with syntaxin-2 and is involved in the terminal step of cytokinesis in mammalian cells. VAMP8 is the major vesicular v-SNARE of zymogen granules from pancreatic acinar cells and has been shown to be a general vesicular SNARE for regulated exocytosis of the exocrine system. For platelet release reaction VAMP8 is the primary v-SNARE.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)