immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Descripción general
Anti-VP16 is produced in rabbit using a synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue. Whole antiserum is fractionated and further purified by ionexchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Inmunógeno
synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue.
Aplicación
Anti-VP16 recognizes VP16 fusion proteins by immunoblotting and immunoprecipitation. It is used to study the effect of sialic acid on herpes simplex virus type 1 envelope glycoproteins.It is also used to study if self-association of lymphocytic choriomeningitis virus nucleoprotein is mediated by its N-terminal region and is not required for its anti-interferon function.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Journal of virology, 80(23), 11589-11597 (2006-09-22)
Following infection, the physical state of linear herpes simplex virus (HSV) genomes may change into an "endless" or circular form. In this study, using Southern blot analysis of the HSV genome, we provide evidence that immediate-early protein ICP4 is involved
The Journal of physiology, 582(Pt 3), 1277-1287 (2007-04-28)
The effects of exercise on skeletal muscle are mediated by a coupling between muscle electrical activity and gene expression. Several activity correlates, such as intracellular Ca(2+), hypoxia and metabolites like free fatty acids (FFAs), might initiate signalling pathways regulating fibre-type-specific
Journal of virology, 81(8), 3731-3739 (2007-01-19)
Herpes simplex virus type 1 (HSV-1) envelope proteins are posttranslationally modified by the addition of sialic acids to the termini of the glycan side chains. Although gC, gD, and gH are sialylated, it is not known whether sialic acids on
Health care's future.
R J Nowak
Ohio medicine : journal of the Ohio State Medical Association, 87(5), 229-230 (1991-05-01)
The biological function of proteins may be predicted by identification of their interacting partners, and one of the major goals of the postgenomic era is the mapping of protein interaction networks. Membrane proteins are of particular interest because of their
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
