SRP2148

EF1α, GST tagged human

recombinant, expressed in E. coli, ≥85% (SDS-PAGE)

• Sinónimos: CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, LENG7, PTI1, eEF1A-1
• UNSPSC Code: 12352200
• NACRES: NA.26

Propiedades

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥85% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~77.3 kDa
• packaging: pkg of 10 μg
• concentration: 350 μg/mL
• color: colorless to clear
• NCBI accession no.: NM_001402
• UniProt accession no.: P68104
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EEF1A1(1915)

Descripción

Biochem/physiol Actions

The alpha subunit of elongation factor-1 (EEF1A) is involved in the binding of aminoacyl-tRNAs to 80S ribosomes. During the process, GTP is hydrolyzed into GDP. To perform this function, EEF1A has domains that bind guanine nucleotides, 80S ribosomes, and aminoacyl-tRNAs. Also, EEF1A interacts with the beta subunit of EEF1 to exchange bound GDP for GTP. Multimolecular complexes involving the eukaryotic elongation factor 1A (eEF1A) have been suggested to play an important role in the channeling (vectorial transfer) of tRNA during protein synthesis HIV-1 matrix protein (MA) was found to interact with elongation factor 1-α (EF1α), an essential component of the translation machinery that delivers aminoacyl-tRNA to ribosomes. The interaction between MA and EF1α impairs translation in vitro, a result consistent with a previously proposed model in which inhibition of translation by the accumulation of Gag serves to release viral RNA from polysomes, permitting the RNA to be packaged into nascent virions. It has been identified that the translation elongation factor, eEF1A, and its guanine nucleotide exchange factor, eEF1Bβ, as translationally controlled tumor protein (TCTP)-interacting partners.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable