SRP2082

WT-1 (+KTS) human

recombinant, expressed in insect cells, ≥60% (SDS-PAGE)

• Código UNSPSC: 12352200
• NACRES: NA.26

Propiedades

• origen biológico: human
• recombinante: expressed in insect cells
• Ensayo: ≥60% (SDS-PAGE)
• Formulario: frozen liquid
• envase: pkg of 5 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• concentración: 150 μg/mL
• color: clear colorless
• Nº de acceso NCBI: NM_024425
• Nº de acceso UniProt: P19544
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... WT1(7490)

Descripción

Acciones bioquímicas o fisiológicas

WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator.

Forma física

Clear and colorless frozen liquid solution

Nota de preparación

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable