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SRP0208

Sigma-Aldrich

PRL-1 (2-173) Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Sinónimos:

PRL-1, PTP4A1, Protein tyrosine phosphatase type IVA, member 1, Protein-tyrosine phosphatase of regenerating liver 1

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About This Item

Código UNSPSC:
51111800
NACRES:
NA.32

origen biológico

human

recombinante

expressed in E. coli

Ensayo

≥90% (SDS-PAGE)

Formulario

aqueous solution

mol peso

19.8 kDa

envase

pkg of 50 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

concentración

>0.02 mg/mL

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... PTP4A1(7803)

Descripción general

Human PRL 1 (GenBank Accession No. BC023975), (amino acids 2-173) with N-terminal HIS tag, MW = 19.8 kDa, expressed in an E. coli expression system.
Protein-tyrosine-phosphatase of regenerating liver 1 (PRL1), also known as protein tyrosine phosphatase type IVA, member 1 (PTP4A1), is encoded by the gene mapped to human chromosome 6q12. The encoded protein is a member of a small class of prenylated protein tyrosine phosphatases (PTPs). It is characterized by a PTP domain and a specific C-terminal prenylation motif. PRL1 is mainly localized to the cell membrane and the endoplasmic reticulum depending on its C-terminal prenylation.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Acciones bioquímicas o fisiológicas

Protein-tyrosine-phosphatase of regenerating liver 1 (PRL1) or protein tyrosine phosphatase type IVA, member 1 (PTP4A1) regulates cancer-related signaling pathways, which are downstream of epidermal growth factor receptor (EGFR) pathway and stimulates cell proliferation, invasion and migration. PRL1 acts as a potential therapeutic target and predictor for patient survival in prostate cancer (PCa) including those with castration-resistant prostate cancer (CRPC). PRL1 is essential for normal mitotic process. Elevated expressions of prenylated protein tyrosine phosphatases (PTPs) in mammalian cells conferred a transformed phenotype, which might be involved in the tumorigenesis.

Definición de unidad

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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The gene encoding human nuclear protein tyrosine phosphatase, PRL-1. Cloning, chromosomal localization, and identification of an intron enhancer.
Peng Y, et al.
The Journal of Biological Chemistry, 273(27), 17286-17295 (1998)
Identification of PRL1 as a novel diagnostic and therapeutic target for castration-resistant prostate cancer by the Escherichia coli ampicillin secretion trap (CAST) method.
Shinmei S
Urologic Oncology, 32, 769-778 (2014)
Jing Wang et al.
The Journal of biological chemistry, 277(48), 46659-46668 (2002-09-18)
PRL-1 is one of three closely related protein-tyrosine phosphatases, which are characterized by C-terminal farnesylation. Recent reports suggest that they are involved in the regulation of cell proliferation and transformation. However, their biological function has not yet been determined. Here

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