SAB4200319

Anti-PTPRA antibody produced in rabbit

~1.0 mg/mL

• Sinónimos: Anti-LRP, Anti-PTPA, Anti-PTPRL2, Anti-PTPase-α, Anti-Tyrosine protein phosphatase receptor type α
• Código UNSPSC: 12352203
• NACRES: NA.44

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~90 kDa
• reactividad de especies: mouse, human
• concentración: ~1.0 mg/mL
• técnicas: indirect immunofluorescence: 5-10 μg/mL using paraformaldehyde fixed HUVEC cells.; western blot: 0.25-0.5 μg/mL using lysates of NIH-3T3 or PC12 cells.
• Nº de acceso UniProt: P18433
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PTPRA(5786) ; mouse ... PTPRA(19262)

Descripción

Descripción general

PTPRA is a transmembrane phosphatase that regulates cell proliferation, cell adhesion, integrin signaling, and growth factor response. This phosphatase also activates proteins of the Src family. PTPRA inhibits cell growth in breast cancer and contributes to tumorigenesis in colon carcinoma . Anti-PTPRA antibody is specific for human and mouse PTPRA. In immunoblotting, detection of the PTPRA band is specifically inhibited by the immunizing peptide.

Protein tyrosine phosphatase alpha (PTPRA) is a transmembrane phosphatase with a relatively short, highly glycosylated extracellular domain, a transmembrane domain, and a tandem phosphatase domain. The amino-terminal domain predominantly mediates catalytic activity, whereas the carboxyterminal domain serves as a regulatory subunit.

Inmunógeno

synthetic peptide corresponding to an internal region of human PTPRA, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.

Aplicación

Anti-PTPRA antibody is suitable for use in western blot (0.25-0.5 μg/mL using lysates of NIH-3T3 or PC12 cells) and indirect immunofluorescence (5-10 μg/mL using paraformaldehyde fixed HUVEC cells).

Anti-PTPRA antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Acciones bioquímicas o fisiológicas

Protein tyrosine phosphatase alpha (PTPRA) is implicated in several signaling pathways, including insulin receptor signaling, cellular transformation and cell spreading and migration. It regulates cell proliferation, cell adhesion, integrin signaling, and growth factor response. PTPRA inhibits cell growth in breast cancer and contributes to tumorigenesis in colon carcinoma.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable