SAB4200031

Anti-TNPO2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-Importin 3, Anti-Karyopherin beta-2b, Anti-Transportin-2
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~95 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over expressing human TNPO2; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human TNPO2; western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human TNPO2
• UniProt accession no.: O14787
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TNPO2(30000)

Descripción

General description

Transportin 2 (TNPO2 or TRN2) is a member of the karyopherin family of transport receptors. This protein exists in two isoforms TRN2a and TRN2b. The TNPO2 gene is located on human chromosome 19p13.2.

Specificity

Anti-TNPO2 (C-terminal) recognizes human TNPO2 isoform b.

Application

Anti-TNPO2 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Transportin 2 (TNPO2) is involved in a novel nuclear protein import and export pathway. This protein specifically binds to M9-containing proteins such as heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) as well as the nucleocytoplasmic shuttling sequence (HNS) region of HuR, but not to the classical nuclear localization signal (NLS)-containing proteins. TNPO2 protein interacts with a similar set of RNA-binding proteins in an RAs-related nuclear protein (Ran)- guanosine triphosphate (GTP) sensitive manner.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable