R5509
Nde I from Neisseria denitrificans
Restriction Enzyme
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About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
Productos recomendados
grado
for molecular biology
Formulario
buffered aqueous glycerol solution
concentración
10,000 units/mL
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
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Especificidad
Recognition sequence: 5′-CA/TATG-3′
Cutting results: a 2-10-fold Nde I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 60 °C for 15 minutes.
Cutting results: a 2-10-fold Nde I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 60 °C for 15 minutes.
Aplicación
NdeI is a restriction endonuclease used in molecular biology methods to cleave DNA at the recognition site 5′-CA/TATG-3′, generating fragments with 5′-cohesive ends.
Otras notas
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Definición de unidad
One unit is the enzyme activity that completely cleaves 1 mg λDNA in 1 hr. at 37 °C in a total volume of 25 mL of restriction endonuclease buffer SH.
Forma física
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 1 mM dithioerythritol, 0.02% polydocanol, 0.01% gelatine, 50% glycerol (v/v) at 4 °C.
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Vega Masignani et al.
The Journal of experimental medicine, 197(6), 789-799 (2003-03-19)
Sepsis and meningitis caused by serogroup B meningococcus are devastating diseases of infants and young adults, which cannot yet be prevented by vaccination. By genome mining, we discovered GNA1870, a new surface-exposed lipoprotein of Neisseria meningitidis that induces high levels
NdeI: a restriction endonuclease from Neisseria denitrificans which cleaves DNA at 5'-CATATG-3' sequences.
R J Watson et al.
FEBS letters, 150(1), 114-116 (1982-12-13)
Cynthia L Richard-Fogal et al.
Journal of bacteriology, 190(10), 3489-3493 (2008-03-11)
The system I cytochrome c biogenesis pathway requires CcmD, a small polypeptide of 69 residues in Escherichia coli. Here it is shown that CcmD is a component of the CcmABC ATP-binding cassette transporter complex. CcmD is not necessary for the
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
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