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P7338

Protease from HIV-1

Name: Protease from HIV-1
Brand: SIGMA

recombinant, expressed in E. coli, buffered aqueous solution, suitable for cleaving HIV substrate III (Yields 2 peaks which elute before the substrate peak when analyzed by reversed phase HPLC.)

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About This Item

Número de CAS:

9001-92-7

MDL number:

MFCD00241889

UNSPSC Code:

12352200

recombinant

expressed in E. coli

form

buffered aqueous solution

suitability

suitable for cleaving HIV substrate III (Yields 2 peaks which elute before the substrate peak when analyzed by reversed phase HPLC.)

shipped in

dry ice

storage temp.

−70°C

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Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Physical form

Solution in dilute HCl, pH 1.6.

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Cloning, expression and purification of a recombinant poly-histidine-linked HIV-1 protease.

A Leuthardt et al.

FEBS letters, 326(1-3), 275-280 (1993-07-12)

The gene coding for the HIV-1 protease was cloned in an Escherichia coli expression vector adding three-histidine codons to the amino and carboxy terminus of the protease sequence. Expression of the protease from this construct led to the accumulation of

Autoprocessing of HIV-1 protease is tightly coupled to protein folding.

J M Louis et al.

Nature structural biology, 6(9), 868-875 (1999-08-31)

In the Gag-Pol polyprotein of HIV-1, the 99-amino acid protease is flanked at its N-terminus by a transframe region (TFR) composed of the transframe octapeptide (TFP) and 48 amino acids of the p6pol, separated by a protease cleavage site. The

Expression of cartilage-derived morphogenetic protein in human intervertebral discs and its effect on matrix synthesis in degenerate human nucleus pulposus cells.

Christine L Le Maitre et al.

Arthritis research & therapy, 11(5), R137-R137 (2009-09-17)

Loss of intervertebral disc (IVD) matrix and ultimately disc height as a result of 'degeneration' has been implicated as a major cause of low back pain (LBP). The use of anabolic growth factors as therapies to regenerate IVD matrix, hence

A single residue, arginine 65, is critical for the functional interaction of leukocyte-associated inhibitory receptor-1 with collagens.

Xiaobin Tang et al.

Journal of immunology (Baltimore, Md. : 1950), 182(9), 5446-5452 (2009-04-22)

ITIM-containing receptors play an essential role in modulating immune responses. Leukocyte-associated inhibitory receptor (LAIR)-1, also known as CD305, is an ITIM-containing inhibitory receptor, expressed by all leukocytes, that binds collagens. In this article, we investigate the effect of a conservative

Retinoic acid-treated pluripotent stem cells undergoing neurogenesis present increased aneuploidy and micronuclei formation.

Rafaela C Sartore et al.

PloS one, 6(6), e20667-e20667 (2011-06-16)

The existence of loss and gain of chromosomes, known as aneuploidy, has been previously described within the central nervous system. During development, at least one-third of neural progenitor cells (NPCs) are aneuploid. Notably, aneuploid NPCs may survive and functionally integrate

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