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P2289

Endoproteinase Lys-C from Lysobacter enzymogenes

lyophilized powder

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Número CAS:
72561-05-8
UNSPSC Code:
12352204
EC Number:
276-716-4
MDL number:
MFCD02253020
Número CE:
3.4.21.50 (BRENDA, IUBMB)
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form

lyophilized powder

Quality Segment

200

analyte chemical class(es)

amino acids

packaging

vial of ≥3.0 units

storage temp.

2-8°C

General description

Endoproteinase Lys-C is an enzyme that preferentially cleaves at the carboxyl side of lysine residues. [1]

Application

Endoproteinase Lys-C from Lysobacter enzymogenes is useful in the determination of primary structures of proteins. [1] It has been used in a study to investigate the evidence for trisulfide bonds in a recombinant variant of a human IgG2 monoclonal antibody. [2]
Endoproteinase Lys-C has been used for the digestion monoclonal antibodies for disulfide bond assignment. [3]

Biochem/physiol Actions

Endoproteinase Lys-C inactivates the enzyme inositol monophosphatase by cleaving it at a single site directly after Lys 36.

Other Notes

One unit will hydrolyze 1.0 μmole of N-p-tosyl-Gly-Pro-Lys p-nitroanilide per min at pH 7.7 at 25 °C.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

wgk

WGK 1

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

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Questions

1–4 of 4 Questions  

  1. Is the protease Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289, active in the presence of detergents?

    1 answer

    1. This enzyme is active in the presence of 1% Triton X-100 and also 0.1% SDS.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What is the molecular weight of Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289?

    1 answer

    1. The molecular weight is 30,000 Daltons (30 kDa).

      Helpful?

  4. What amount of Endoproteinase Lys-C from Lysobacter enzymogenes, Product P2289, is present in the 3 unit package size?

    1 answer

    1. This product is prepared from material with a specific activity of 150 units per mg. Therefore there is approximately 20 micrograms per vial.

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