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OGS2806

PSF-OXB20-NH2-10HIS-EKT - N-TERMINAL 10HIS TAG BACTERIAL PLASMID

Name: PSF-OXB20-NH2-10HIS-EKT - N-TERMINAL 10HIS TAG BACTERIAL PLASMID
Brand: SIGMA

plasmid vector for molecular cloning

Sinónimos:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Código UNSPSC:

12352200

NACRES:

NA.85

Este producto está descatalogado. Póngase en contacto con el Servicio técnicopara asistencia

recombinante

expressed in E. coli

etiqueta

10-His tagged

Formulario

buffered aqueous solution

mol peso

size 4982 bp

selección de bacterias

kanamycin

Origen de replicación

pUC (500 copies)

Escisión peptídica

EKT

Ubicación de la etiqueta en el péptido

N-terminal

Promotor

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

gen reportero

none

Condiciones de envío

ambient

temp. de almacenamiento

−20°C

Descripción general

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains an n-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

Secuencia

To view sequence information for this product, please visit the product page

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Producto relacionado

Referencia del producto

Descripción

Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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