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Merck

N7265

Sigma-Aldrich

Nitrate Reductase (NAD[P]H) from Aspergillus niger

lyophilized powder, ≥300 units/g solid

Sinónimos:

NAD(P)H:Nitrate oxidoreductase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

specific activity

≥300 units/g solid

shipped in

wet ice

storage temp.

−20°C

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General description

Nitrate reductase (NAD[P]H) is expressed in variety of organisms including bacteria, fungi, algae, and higher plants which have capacity to use nitrate as their sole source of nitrogen.Experimental studies show that nitrate reductase (NAD[P]H) is 180,000Daltons.

Biochem/physiol Actions

Nitrate Reductase (NAD[P]H) catalyzes the reduction of nitrate to nitrite. In addition, it also catalyzes the reduction of NADPH-cytochrome c, FADH2-nitrate. Nitrate reductase also has reduced methyl viologen-nitrate reductase activities.

Caution

light and moisture sensitive

Unit Definition

One unit will reduce 1.0 μmole of nitrate per min in the presence of β-NADPH at pH 7.5 at 25 °C.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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P Hilliquin et al.
Arthritis and rheumatism, 40(8), 1512-1517 (1997-08-01)
Nitric oxide (NO) is a free radical involved in inflammation and immune reactions. The presence of NO is usually assessed by assaying its degradation products, nitrite and nitrate. NO binds to thiol-containing proteins to form S-nitrosoproteins (S-NP). The aim of
Nilesh Dharajiya et al.
The Journal of allergy and clinical immunology, 119(3), 646-653 (2007-03-06)
Ragweed extract (RWE) contains NADPH oxidases that induce oxidative stress in the airways independent of adaptive immunity (signal 1) and augment antigen (signal 2)-induced allergic airway inflammation. To test whether inhibiting signal 1 by administering antioxidants inhibits allergic airway inflammation
E Duncanson et al.
Molecular & general genetics : MGG, 236(2-3), 275-282 (1993-01-01)
Eleven green individuals were isolated when 95000 M2 plants of barley (Hordeum vulgare L.), mutagenised with azide in the M1, were screened for nitrite accumulation in their leaves after nitrate treatment in the light. The selected plants were maintained in
E Fernández et al.
Biochimica et biophysica acta, 657(1), 1-12 (1981-01-15)
In vitro complementation of the soluble assimilatory NAD(P)H-nitrate reductase (NAD(P)H:nitrate oxidoreductase, EC 1.6.6.2) was attained by mixing cell-free preparations of Chlamydomonas reinhardii mutant 104, uniquely possessing nitrate-inducible NAD(P)H-cytochrome c reductase, and mutant 305 which possesses solely the nitrate-inducible FMNH2- and
R L Warner et al.
Plant physiology, 91, 947-953 (1989-01-01)
Barley (Hordeum vulgare L.) has NADH-specific and NAD(P)H-bispecific nitrate reductase isozymes. Four isogenic lines with different nitrate reductase isozyme combinations were used to determine the role of NADH and NAD(P)H nitrate reductases on nitrate transport and assimilation in barley seedlings.

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