N214

Neutrophil Activating Peptide-2 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinónimos: NAP-2
• Número MDL: MFCD00241917
• Código UNSPSC: 51111800

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Ensayo: >97% (SDS-PAGE)
• Formulario: lyophilized powder
• potencia: 0.1-0.3 μg/mL ED₅₀ (cytochalasin B treated human neutrophils); 0.1-0.5 ng/mL ED₅₀ (BaF/3 hCXCR-2 transfected cells)
• mol peso: ~7.6 kDa
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)
• técnicas: cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• Nº de acceso UniProt: P02775
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... PPBP(5473)

Descripción

Acciones bioquímicas o fisiológicas

Neutrophil Activating Peptide-2, a member of the CXC chemokine family, chemoattracts and activates neutrophils and also binds CXCR-2. NAP-2 is proteolytically processed C-terminal fragments of platelet basic protein (PBP), which is found in the α-granules of human platelets.

Forma física

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Nota de análisis

The biological activity is measured by the induction of myeloperoxidase release from cytochalasin B treated human neutrophils.