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MSANGERV

Sigma-Aldrich

Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Mouse, Virus Format

Sinónimos:

Arrayed CRISPR library, Sanger CRISPR library

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About This Item

UNSPSC Code:
41105904

Quality Level

200

packaging

pkg of 10 μL (384-well plate)

concentration

1x106  VP/ml (via p24 assay)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

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General description

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

Application

Functional Genomics/Screening /Target Validation

Features and Benefits

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP

Additional Features
  • Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Packaging

384 well plates

Components

Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones


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Physical form

10μl of lentivirus at ≥106 particles/ml in ~120×384 well plates

Other Notes

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Recommended products

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Human, Virus Format

Legal Information

CRISPR Use License Agreement

Lentiviral License Agreement

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al
Scientific Reports, 7 (2017)

Artículos

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Protocolos

Pasos y método de secuenciación de Sanger

Conozca los pasos de la secuenciación de Sanger o método de terminación de la cadena y cómo funciona la secuenciación de ADN y cómo leer los resultados de la secuenciación de Sanger con precisión para su investigación.

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico