neurological disorders; aging/geriatric diseases; cancer
storage temp.
−20°C
General description
Adenosine Monophosphate (AMP), also known as 5′-adenylic acid, consists of adenine, ribose and phosphate. AMP can be produced during ATP synthesis by the enzyme adenylate kinase by combining two ADP molecules, as a result of the hydrolysis of ATP/ADP, or when RNA is broken down. AMP plays an important role in many cellular metabolic processes, such as Ca2+ signaling, cell migration, cytokine secretion etc. AMP is an activator of AMP-activated protein kinase, which regulates glucose uptake, fatty acid uptake, fatty acid oxidation etc. AMP levels can be measured by luciferase/luciferin mediated assays. However, luciferase signal is unstable and luminescent equipment is generally not available in most laboratories.
Features and Benefits
AMP Assay Kit:
Compatible with high-throughput handling systems.
Sensitive, detecting AMP levels lower than 10 μM in various sample types.
Suitability
The kit is suitable for the measurement of AMP levels in various cell (adherent or suspension cells: e.g. HeLa, HEK239, Jurkat cells) and tissue lysates (e.g. liver, kidney, etc.), the study of the regulation of AMPK by AMP and for the mechanistic study of key cellular processes such as Ca2+ signaling, glucose uptake, lipid uptake, etc.
Principle
In this assay, AMP is converted to pyruvate in the presence of pyrophosphate and phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at A570 nm. The absorbance is proportional to the amount of AMP present in samples.
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