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MAK266

Sigma-Aldrich

Triglyceride Quantification Colorimetric/Fluorometric Kit

Triglyceride Quantification Colorimetric/Fluorometric Kit
1 of 3 reviewers received a sample product or took part in a promotion

sufficient for 100 colorimetric or fluorometric tests

Sinónimos:

TG Quantification Kit

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1 KIT
US$ 872,00

US$ 872,00

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1 KIT
US$ 872,00

About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

US$ 872,00

PRECIOS SIN IMPUESTOS NACIONALES
Check Cart for Availability
Solicitar un pedido a granel

método de detección

colorimetric
fluorometric

enfermedades relevantes

neurological disorders; cardiovascular diseases

temp. de almacenamiento

−20°C

Descripción general

The new Triglyceride Quantification Kit, MAK564, is now available! Triglycerides (TG) are the main constituents of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as an energy source.[1][2] TG is broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways.[3] High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.[4]

Idoneidad

Suitable for use with serum, plasma, and other biological fluids, and tissue and cell culture samples.

Principio

The Triglyceride Quantification Kit provides a sensitive, easy assay to measure TG concentration in a variety of samples. In this assay, TG are converted to free fatty acids and glycerol. The glycerol is then oxidized to generate a colorimetric (570 nm)/fluorometric (lex = 535 nm/lem = 587 nm) product. The kit is sensitive to detect 2 pmole–10 nmole (2–10,000 mM range) of triglyceride in various samples. The kit also detects monoglycerides and diglycerides.

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H317,H334

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

188.6 °F - closed cup

Punto de inflamabilidad (°C)

87 °C - closed cup

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
8K18K06220
8H26K06220
8H11K06220
8F20K06220
8A17K06220

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Clinical biochemistry and hematology.
The laboratory rabbit, guinea pig, hamster, and other rodents., 57-116 (2012)
Pingping Luo et al.
Acta pharmaceutica Sinica. B, 11(3), 668-679 (2021-03-30)
Alcoholic liver disease (ALD) causes insulin resistance, lipid metabolism dysfunction, and inflammation. We investigated the protective effects and direct regulating target of S-allylmercaptocysteine (SAMC) from aged garlic on liver cell injury. A chronic ethanol-fed ALD in vivo model (the NIAAA model)
The role of triglycerides in atherosclerosis.
Talayero, Beatriz G., and Frank M. Sacks.Talayero, Beatriz G and Frank M S
Current Cardiology Reports, 13(6), 544-544 (2011)
Glyceroneogenesis and the triglyceride/fatty acid cycle.
Reshef L, et al.
The Journal of Biological Chemistry, 278(33), 30413-30416 (2003)
L Vogel et al.
Journal of dairy science, 103(8), 7431-7450 (2020-06-02)
Rations including high amounts of corn silage are currently very common in dairy production. Diets with corn silage as forage source result in a low supply of essential fatty acids, such as α-linolenic acid, and may lead to low conjugated
Ver todos los artículos relacionados
3 Reviews This action will navigate to reviews.

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Can ethanol or isopropanol (IPA) be used instead of water for liver tissue sample preparation with the Triglyceride Quantification Colorimetric/Fluorometric Kit (MAK266)?

    1 answer

    1. Based on the guidelines, only water is an appropriate solvent for samples in this assay. Ethanol and isopropanol are not suitable solvents for this assay.

      Helpful?

  4. Is liver tissue extracted using RIPA buffer and without NP-40 compatible with the MAK266 Triglyceride Quantification Colorimetric/Fluorometric Kit?

    1 answer

    1. It is recommended to use NP-40 or similar non-ionic detergents instead of RIPA, due to the presence of harsh detergents in RIPA.

      Helpful?

Reviews

1 of 3 reviewers received a sample product or took part in a promotion

Active Filters

  1. Pittsburgh, PA
    • Review 1
    • Votes 8
    1 out of 5 stars.

    Terrible kit!

    The protocol is not well-written. I looked for several literatures who used this kit and found inconsistent data for control samples. Even the technical staffs of Sigma could not satisfy my questions and I strongly discourage other shoppers to use this.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for reaching out and sharing your review with us. We are sorry to hear about your experience with this product and is not what we would expect. Our records show your case was resolved as there were no additional follow up questions. We have provided your feedback to our Product Management team for further review.

  2. 2 Ratings-Only Reviews

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