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MAK085

Sigma-Aldrich

Kit de análisis de la peroxidación lipídica (MDA)

sufficient for 100 colorimetric or fluorometric tests

Sinónimos:

Análisis de sustancias reactivas del ácido tiobarbitúrico, Ensayo MDA, Ensayo TBARS

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

control farmacológico

regulated under CDSA - not available from Sigma-Aldrich Canada

método de detección

colorimetric
fluorometric

enfermedades relevantes

endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases

temp. de almacenamiento

−20°C

Descripción general

La peroxidación lipídica es la degradación de los lípidos que se produce como consecuencia del daño oxidativo y es un marcador útil del estrés oxidativo. Los lípidos poliinsaturados son sensibles a un ataque oxidativo, normalmente por especies de oxígeno reactivo, lo que da lugar a una reacción en cadena bien definida con la producción de productos finales como el malondialdehído (MDA). La peroxidación lipídica puede contribuir a la patología de muchas enfermedades, como la aterosclerosis, la diabetes y el Alzheimer.

Aplicación

El kit de análisis de la peroxidación de lípidos (MDA) se ha utilizado para determinar los niveles de malondialdehído (MDA).[1][2]

Idoneidad

Es idóneo para medir el malondialdehído (MDA) en una variedad de muestras: tejidos, células y plasma

Principio

En este kit, la peroxidación de lípidos se determina mediante la reacción del MDA con el ácido tiobarbitúrico (TBA) para formar un producto colorimétrico (532 nm) o fluorométrico (λex = 532/λem = 553 nm), proporcional al MDA presente.

Producto relacionado

Referencia del producto
Descripción
Precios

92210

Timestrip Plus™ 0 °C

Palabra de señalización

Danger

Frases de peligro

H225,H302,H314,H411

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1C

Código de clase de almacenamiento

3 - Flammable liquids

Punto de inflamabilidad (°F)

57.2 °F - closed cup

Punto de inflamabilidad (°C)

14 °C - closed cup

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
8L03K07390
8A20K07390
8F15K07390
8H25K07390
8C28K07390

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Questions

1–7 of 7 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is the MAK085-1KT Lipid Peroxidation (MDA) Assay Kit compatible with tissue samples stored in 4% paraformaldehyde PBS?

    1 answer

    1. The assay has not been tested in PFA-fixed or methanol-fixed samples, and it is not predicted that the kit would work robustly or consistently in these samples.

      Helpful?

  4. Is MAK085 suitable for use on plant samples?

    1 answer

    1. The MAK085 kit has never been tested on plant samples, and thus there is no optimized protocol for plant samples. This kit has only been validated for tissue, cells, serum, or plasma samples. It is possible to adapt the protocol for plant samples and test it, but there is no guarantee that it will work.

      Helpful?

  5. We are planning an MDA assay using mouse tissue. Should I avoid cryopreservation of the samples? How should we store the samples if they cannot be assayed immediately?

    1 answer

    1. Whenever possible, It is recommended to use fresh samples. If sufficient fresh samples are not available, samples may be flash frozen in liquid N2 and stored at -80C. A storage time frame has not been determined. Cryopreservation of samples should be avoided as this may affect the integrity biomolecules and contribute to oxidative stress.

      Helpful?

  6. Can you please send us a protocol how to use MDA Assay kit (MAK085-1KT). We have ordered a kit with lot# 8L03K07390. Thanks

    1 answer

    1. Please see the link below to review the product datasheet which includes the protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/779/633/mak085pis-ms.pdf

      Helpful?

  7. MDA assay kit 구성품 중 TBA(Catalog Number MAK085D)이 4 bottles 있다고 명시되어있는데, 1 bottle 당 몇 g이 들어가있는건가요?

    1 answer

    1. Each bottle contains 250 mg of Thiobarbituric Acid (TBA).

      Helpful?

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