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K4002
Krebs-Ringer Bicarbonate Buffer
With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
Sinónimos:
Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution
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10 X 1 L
US$ 303,00
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10 X 1 L
US$ 303,00
About This Item
Código UNSPSC:
12161702
NACRES:
NA.75
Productos recomendados
Nivel de calidad
Formulario
powder
técnicas
cell culture | mammalian: suitable
pH
6.1-6.7
solubilidad
water: 9.5 g/L, clear
temp. de almacenamiento
2-8°C
Descripción general
Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.
Aplicación
Cantidad
Formulated to contain 9.5 grams of powder per liter of medium.
Reconstitución
Supplement with 1.26 g/L sodium bicarbonate.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Protocolos
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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What is the Department of Transportation shipping information for this product?
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How should Product K4002, Krebs-Ringer Bicarbonate Buffer, be dissolved?
1 answer-
Product No. K4002 is listed in a 10X1L package size, meaning that each of the ten containers has enough powder to make 1 Liter of KRB buffer. It can be dissolved in 1 Liter of either water or medium.
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What is the formula of Product K4002, Krebs-Ringer Bicarbonate Buffer (KRB)?
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The formulation for KRB can be found on the data sheet.
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Product K4002 - Krebs-Ringer Bicarbonate Buffer, is without calcium chloride and sodium bicarbonate. In the reconstitution steps, you advice to add 1.26g sodium bicarbonate. Should we also add some calcium chloride? in which amount?
1 answer-
Product K4002 - Krebs-Ringer Bicarbonate Buffer does not contain calcium. The addition of calcium is not required for osmolarity. If you need to have calcium, you can add calcium chloride between 1.25 to 2.5 mM. You may want to check the osmolarity and make sure it is not too high.Kreb Henseleit Buffer has 1.25 mM calcium chlorideKreb's Buffer has 2.5 mM calcium chlorideKreb' Ringer Superfusion solution contains 2.0 mM calcium chloride.Please see the attached document for different Krebs' buffer recipes.
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