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HT8028

Malachite Green Solution

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NACRES:
NA.47
UNSPSC Code:
41116121
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form

solution

Quality Segment

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

technique(s)

microbe id | staining: suitable

application(s)

hematology
histology
microbiology

storage temp.

room temp

Application

Intended for use with Carbol Fuchsin (HT80-1) in Sigma Acid Fast Stain, Procedure HT80. Acts as both a counterstain and a differentiator.

Other Notes

Malachite green oxalate (certified), 1.5%; acetic acid, 10%; and glycerol, 17%, in deionized water.


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Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2

Clase de almacenamiento

8A - Combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

flash_point_c

Not applicable



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Questions

  1. Can the HT8028 Malachite Green solution be used as a spore stain?

    1 answer
    1. The Malachite Green solution (HT8028-250ml and HT80616-500ml) has not been tested for use in various procedures to stain spores. There is a Schaefer and Fulton Modification of the Wirtz Spore stain, which lists the formulation for the Malachite Green solution as simply 5% malachite green solution in water. However, the HT80 procedure lists a different formulation for the Malachite Green solution intended for use in staining Acid Fast Bacillus (AFB), which contains malachite green oxalate, acetic acid, and glycerol in deionized water. Given the difference in formulations and the inclusion of an acid differentiator in the HT802 formulations, no claims can be made that this solution is suitable for staining spores. The solution might work, or it might not.

      A better option might be to order kit 04551-1kt-F, the Schaeffer and Fulton Spore Stain kit, which consists of a 5% solution of Malachite Green as the first component, and a Safranin O solution 0.5% as the second component.

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