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FP0010

Sigma-Aldrich

FluoroProfile™ Protein Quantification Kit

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About This Item

UNSPSC Code:
12352200

shipped in

wet ice

storage temp.

−20°C

Application

Reversible covalent binding to proteins by the fluorophore epicocconone provides a powerful and sensitive method for protein quantification

Features and Benefits

  • Yields an intense fluorescent red on protein binding
  • Binding is completely reversible making the protein suitable for mass spectrometry and functional assays
  • Water soluble proteins may be quantified without using organic solvents
  • A large dynamic range, large Stokes shift and highly sensitive
  • Highly tolerant of substances commonly interfering with protein quantification
  • Biodegradable and environmentally friendly

Other Notes

For the quantification of total protein in solution by the interaction of a unique fluorophore with the amines of polypeptides.

Legal Information

FluoroProfile is a trademark of Sigma-Aldrich Co. LLC

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • P5619Protein Standard, analytical standard, 2 mg/vial BSASDS

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
SLBS2677V
SLBP5200V
SLBN6260V
SLBN3450V
SLBM4707V

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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R P Ubrihien et al.
Archives of environmental contamination and toxicology, 82(4), 520-538 (2022-04-21)
The effects of multigenerational Cu exposure on the freshwater gastropod Isidorella newcombi were investigated. Snails were exposed to a range of treatment-specific Cu concentrations in the parental to F2 generations, and a common Cu concentration in the F3 generation. In
Ondřej Kaplan et al.
PloS one, 11(10), e0163697-e0163697 (2016-10-08)
We describe the production of a highly-active mutant VEGF variant, α2-PI1-8-VEGF121, which contains a substrate sequence for factor XIIIa at the aminoterminus designed for incorporation into a fibrin gel. The α2-PI1-8-VEGF121 gene was synthesized, cloned into a pET-32a(+) vector and
Phillip J L Bell et al.
Journal of the American Chemical Society, 125(31), 9304-9305 (2003-08-02)
Epicocconone represents a new class of natural fluorescent probes based on a polyketide skeleton isolated from the fungus Epicoccum nigrum. Epicocconone is a small, cell permeable natural product with a high molar absorbtivity and a long Stokes' shift that will
Jiaorong Tan et al.
Journal of diabetes research, 2019, 5483946-5483946 (2019-06-22)
This study is aimed at evaluating the effects, functions, and mechanism of HNF1α on hepatic glycolipid metabolism. In this study, free fatty acid- (FFA-) induced steatosis of hepatocyte liver cell LO2 was used as an in vitro model. The methods
Helena A Schmitz et al.
Aquatic toxicology (Amsterdam, Netherlands), 160, 22-30 (2015-01-13)
Metals are accumulated by filter feeding organisms via water, ingestion of suspended sediments or food. The uptake pathway can affect metal toxicity. Saccostrea glomerata were exposed to cadmium through cadmium-spiked suspended sediments (19 and 93μg/g dry mass) and cadmium-enriched phytoplankton
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Questions

1–4 of 4 Questions  

  1. Can FP0010, FluoroProfile® Protein Quantification Kit, be used to stain protein gels?

    1 answer

    1. We have not tried using this kit in that particular application.

      Helpful?

  2. Can I run Product FP0010, FluoroProfile® Protein Quantification Kit, at 37 °C?

    1 answer

    1. We generally test the kit at ambient temperature, but we expect that customers can use this kit at 37 °C.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. I don't have black plates. Can I use white plates with Product FP0010, FluoroProfile® Protein Quantification Kit?

    1 answer

    1. We strongly recommend use of black plates for the fluorescence readings. White plates are more suitable for luminescence and diminished sensitivity with this kit is extremely likely with white plates.

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