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F7378

Sigma-Aldrich

Fluorescein diacetate

Powder

Sinónimos:

3,6-Diacetoxyfluoran, Di-O-acetylfluorescein

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5 G
US$ 253,00
10 G
US$ 448,00
25 G
US$ 945,00
100 G
US$ 2.530,00

US$ 253,00

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5 G
US$ 253,00
10 G
US$ 448,00
25 G
US$ 945,00
100 G
US$ 2.530,00

About This Item

Fórmula empírica (notación de Hill):
C24H16O7
Número de CAS:
596-09-8
Peso molecular:
416.38
Beilstein/REAXYS Number:
62575
EC Number:
209-877-6
MDL number:
MFCD00005062
UNSPSC Code:
12171500
PubChem Substance ID:
24894939
NACRES:
NA.47

US$ 253,00

En stockDetalles

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Nombre del producto

Fluorescein diacetate, used as cell viability stain

Quality Level

200

form

powder

technique(s)

titration: suitable

color

white to faint yellow

mp

200-203 °C (lit.)

solubility

acetone: 25 mg/mL

ε (extinction coefficient)

≥86000 at 488-494 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

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General description

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.[1]

application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.[2][3]

Substrates

Lipase substrate

related product

Referencia del producto
Descripción
Precios

06693

Timestrip Plus™ -20 °C

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000413679
0000413679
0000344082
MKCM9758
0000222188

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Questions

1–6 of 6 Questions  

  1. What are some common applications for Product F7378, Fluorescein diacetate?

    1 answer

    1. This compound is used extensively for the staining of living cells.  It has been used as a viability stain for cultured neurons and plant ways, and in measuring of esterase and cholinesterase activity. See the following references for usage: Fluorometric determination of lipase: Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964. ; Anal. Biochem., Vol. 14, 28 (1966);Fluorescent stain for viable rosette-forming cells: J. Immun. Meth., Vol. 5,305 (1974);Fluorogenic substrate detection of viable pathogenic protozoa: Science, Vol. 227, 435 (1985);Reference Standard in antibody studies: Anal. Biochem., Vol. 9, 474 (1964);Cell viability staining: Staining Procedures, G. Clark, 4th ed., p. 93.Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes, Anal. Chem. 36, 409, 1964.""   "

      Helpful?

  2. After Product F7378, Fluorescein diacetate, has been converted to fluorescein, what are the maximum excitation and emission wavelengths?

    1 answer

    1. The maximum excitation wavelength is 490 nm and the maximum emission wavelength is 526 nm.

      Helpful?

  3. How stable are solutions of Product F7378, Fluorescein diacetate, once prepared?

    1 answer

    1. Based on our experience, stock solutions of fluorescein diacetate at 5 mg/mL in acetone or DMSO can be stored at -20 °C. According to one on-line source, solutions at 5 mg/mL in acetone or ethanol can be stored refrigerated.

      Helpful?

  4. Is Product F7378, Fluorescein diacetate, a fluorescent compound?

    1 answer

    1. No. Only upon hydrolysis of the esters, catalyzed by cellular esterase, the lactone ring opens yielding fluorescein.  Only the fluorescein form is fluorescent.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What solubility information do you have on Product F7378, Fluorescein diacetate?

    1 answer

    1. We have found that it is slightly soluble in water at 0.1%, and in ethanol at 0.3%. It is also soluble in dimethylformamide (DMF).

      Helpful?

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