The activity was found by taking the units/ml of enzyme and dividing it by the mg protein/ml of enzyme. The activity was found in an spectrophometric rate determination assay. The result found that one unit of enzyme will oxidize 1.0 micro mole of formaldehyde to formic acid per minute at pH 7.5 at 37°C.
F1879
Formaldehyde Dehydrogenase from Pseudomonas sp.
lyophilized powder, 1.0-6.0 units/mg solid
Sinónimos:
FDH, Formaldehyde:NAD+ oxidoreductase
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Specific activity:
1.0-6.0 units/mg solid
form
lyophilized powder
specific activity
1.0-6.0 units/mg solid
storage temp.
−20°C
General description
Formaldehyde Dehydrogenase (FDH) belongs to the medium-chain alcohol dehydrogenase family. It is encoded by the fdhA and fdhB gene in Pseudomonas aeruginosa. The FDH sequences of P. aeruginosa and P. putida are conserved and show homology. FDH exists as dimer and tetramer with zinc in their catalytic active site. The monomer corresponds to 350-400 amino-acid residues.
Application
Formaldehyde Dehydrogenase from Pseudomonas sp. has been used:
- For standard curve generation for the demethylation-FDH assay of lysine specific demethylase 1 (LSD1) and in formaldehyde dehydrogenase (FDH) assay.
- As a biosensor for the presence of formaldehyde in pharmaceuticals, waste water, vaccines and industrial products.[1]
- In coupled pectin methyl esterase (PME) enzyme assay.[1]
Biochem/physiol Actions
Formaldehyde dehydrogenase (FDH) is an enzyme that catalyzes the oxidation of formaldehyde to formate with NAD+ as an electron acceptor.Formaldehyde dehydrogenases are involved in the biological detoxification of formaldehyde, a toxic product in the metabolism of compounds like histidine, vanillate etc. They detoxify by converting formaldehyde to carbon-di-oxide. FDH plays a crucial role in regulating protein S-nitrosation in mammalian cells by decomposing S-nitrosoglutathione. They are also involved in the regulation of nitrosothiol homeostasis by catalyzing the reduction of endogenous nitrosylating agent S-nitrosoglutathione (GSNO).
Physical form
Lyophilized powder containing ~70% stabilizers as Mg2+, Ca2+, bovine serum albumin, glycine, and lysine
Other Notes
One unit will oxidize 1.0 μmole of formaldehyde to formic acid per min at pH 7.5 at 37 °C.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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