D9909
Dextran Sucrase from Leuconostoc mesenteroides
lyophilized powder, ≥100 units/mg protein
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| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 10 units | Comprobar disponibilidad del carrito | US$ 584,00 |
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
Biological source:
bacterial (Leuconostoc mesenteroides)
US$ 584,00
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Permítanos ayudarlebiological source
bacterial (Leuconostoc mesenteroides)
Quality Level
form
lyophilized powder
specific activity
≥100 units/mg protein
composition
Protein, ~15% Lowry
solubility
H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
storage temp.
−20°C
1 of 1
Este artículo | |||
|---|---|---|---|
| biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) |
| specific activity ≥100 units/mg protein | specific activity - | specific activity - | specific activity - |
| form lyophilized powder | form powder | form powder | form powder |
| storage temp. −20°C | storage temp. - | storage temp. - | storage temp. - |
| solubility H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow | solubility H2O: soluble 20 mg/mL | solubility H2O: 100 mg/mL, clear, colorless | solubility H2O: 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.
Application
Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.
Dextran Sucrase from Leuconostoc mesenteroides has been used:
- in immobilization on shirasu porous membrane (SPG) for dextran production
- in the enzymatic synthesis of dextran nanoparticles at various pH range
- in the immobilization with hydroxyapatite for dextran production
The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.
Biochem/physiol Actions
Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages.[1] The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad.[2] The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.[3]
Physical form
Lyophilized powder containing dextran, MES buffer salts and CaCl2
Analysis Note
Chromatographically purified
Other Notes
One unit will liberate 1.0 μmole of fructose per min at 37 °C, pH 5.4.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Bioengineering of Leuconostoc mesenteroides glucansucrases that gives selected bond formation for glucan synthesis and/or acceptor-product synt
Kang, H., et al.
RNA, 59, 4148-4155 (2011)
Ah-Rum Yi et al.
Journal of microbiology and biotechnology, 19(8), 829-835 (2009-09-08)
A dextransucrase (LcDS) gene from Leuconostoc citreum HJ-P4 has been amplified and cloned in E. coli. The LcDS gene consists of 4,431 nucleotides encoding 1,477 amino acid residues sharing 63-98% of amino acid sequence identities with other known dextransucrases from
Hongbin Zhang et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 25(12), 2022-2028 (2010-04-01)
We optimized the medium for recombinant dextransucrase expression in engineering strain Escherichia coli BL21 (DE3)/pET28-dexYG by an Orthogonal experiment. After the medium had been decided, we studied the effect of temperature, sucrose concentration and pH value on the yield. The
Enzymatically synthesized dextran nanoparticles and their use as carriers for nutraceuticals
Semyonov D, et al.
Food & Function, 5(10), 2463-2474 (2014)
Marie-Sophie Bounaix et al.
FEMS microbiology letters, 311(1), 18-26 (2010-08-21)
The study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that Weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. In this study, several dextran-producing strains, Weissella cibaria
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| D9909-10UN | 04061833591352 |
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