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Merck

D5042

Sigma-Aldrich

123 bp DNA Ladder

for DNA electrophoresis

Sinónimos:

DNA Ladder for DNA electrophoresis, DNA marker

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About This Item

UNSPSC Code:
41105335
NACRES:
NA.25

Quality Level

form

liquid

usage

100 uses (0.1 mg size)
250 uses (250 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Application

123 bp DNA Ladder has been used as a size marker in random amplified polymorphic DNA-polymerase chain reaction (PCR) analysis. It has also been used as a molecular marker in gel electrophoresis.

Components

Sigma′s 123 bp DNA Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 0.1 mM EDTA.

Other Notes

Contains 35 fragments, 123 - 4182 bp
For optimal resolution, the recommended agarose gel concentration is 1.5%.

related product

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Gene splicing and mutagenesis by PCR-driven overlap extension
Heckman KL and Pease LR
Nature Protocols, 2(4), 924-932 (2007)
Molecular characterization of lactic acid bacteria isolated from beef and stored using vacuum-packaging and advanced vacuum skin packaging systems
Inguane AS, et al.
CYTA-journal of Food (2011)
Straightforward procedure for laboratory production of DNA ladder
Lan VTT, et al.
Journal of Nucleic Acids, 2012 (2012)
Insulin-like growth factor I and II preserve myocardial structure in postinfarct swine
Kotlyar AA, et al.
British Heart Journal, 86(6), 693-700 (2001)
E J Gubbins et al.
The Journal of biological chemistry, 255(18), 8655-8662 (1980-09-25)
The organization and sequence of the rat preprolactin gene has been investigated. Analysis of two different plasmids containing pituitary cDNA inserts has provided the complete 681-nucleotide coding sequence of preprolactin as well as 17 nucleotides preceding the initiation codon and

Artículos

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Contenido relacionado

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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