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D4293

3,3′-Diaminobenzidine

SIGMAFAST, chromogenic, tablet

Sinónimos:

DAB tablets

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A ustedes/SKUDisponibilidadPrecio
5 sets
Comprobar disponibilidad del carrito
US$ 71,90
50 sets
Comprobar disponibilidad del carrito
US$ 353,00
5 x 50 set
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US$ 1.300,00

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Número CAS:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:

US$ 71,90

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Nombre del producto

SIGMAFAST 3,3′-Diaminobenzidine tablets, tablet, To prepare 5 mL

Quality Segment

form

tablet

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

Application

SIGMAFAST 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue, thymus, dental chronic periapical lesions and gall bladder sections.
Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Preparation Note

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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D4168D8001D12384
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

tablet

form

tablet

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

-


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signalword

Danger

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



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Questions

1–2 of 2 Questions  
  1. Hello, your product D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for how many months after dissolving in 5ml of distilled water?

    1 answer
    1. This product is a set of two tablets - one DAB tablet and one Urea Hydrogen Peroxide tablet. When prepared according to instructions, the solution should be used within one hour for best results.

      Helpful?

  2. Hello, Can https://www.sigmaaldrich.com/US/en/product/sigma/d4293?icid=sharepdp-clipboard-copy-productdetailpage this product be used to develop western blots? Thank you, Regards Vanda

    1 answer
    1. D4293, SIGMAFAST™ 3,3′-Diaminobenzidine tablets, can be used for WB on PVDF membranes, please see attached reference paper stating below: "The PVDF was then collected and rinsed 3 times for 5–10 min each wash with TBS. The blot was then developed appropriately with 3',3-Diaminobenzidine tablets for developing peroxidase (D4293 Sigma-Aldrich) and BCIP/NBT for alkaline phosphatase (B5655 Sigma-Aldrich) and the blot was imaged."Scientific Neurogenic marker expression in differentiating human adipose derived adult mesenchymal stem cells Neus Gomila Pelegri1,2, Bruce K. Milthorpe1 , Catherine A. Gorrie2 , Jerran Santos1. Stem Cell Investig 2023;10:7

      Helpful?

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