The molecular weight for this product is not determined. However, other sources report a molecular weight of 24,000 daltons for Diaphorase from Clostridium.
D2197
Diaphorase from Clostridium kluyveri
recombinant, expressed in E. coli, Animal-component free
Sinónimos:
Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
100 UNITS
US$ 105,00
300 UNITS
US$ 287,00
500 UNITS
US$ 367,00
Seleccione un Tamaño
Cambiar Vistas
100 UNITS
US$ 105,00
300 UNITS
US$ 287,00
500 UNITS
US$ 367,00
About This Item
Productos recomendados
recombinante
expressed in E. coli
Nivel de calidad
Formulario
solid
actividad específica
>=30 units/mg protein (biuret)
temp. de almacenamiento
−20°C
Descripción general
Research area: Cancer
Aplicación
Diaphorase from Clostridium kluyveri has been used:
- in lactate dehydrogenase assay in gill cells.[1]
- impregnated on the strip for formate dehydrogenase assay.[2]
- in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
- for semiquinone generation during benzoquinone reduction.[3]
- for evaluation of glucose uptake in cultured muscle cells.[4]
- in lactate dehydrogenase cytotoxicity assay.[5]
Acciones bioquímicas o fisiológicas
Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.
Definición de unidad
One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.
Forma física
Supplied as a lyophilized powder containing trehalose.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Apor Veres-Székely et al.
International journal of molecular sciences, 23(4) (2022-02-27)
Fibroblasts play a central role in diseases associated with excessive deposition of extracellular matrix (ECM), including idiopathic pulmonary fibrosis. Investigation of different properties of fibroblasts, such as migration, proliferation, and collagen-rich ECM production is unavoidable both in basic research and
Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
Claudio F Gonzalez et al.
The Journal of biological chemistry, 280(24), 22590-22595 (2005-04-21)
Most bacteria contain soluble quinone-reducing flavoenzymes. However, no biological benefit for this activity has previously been demonstrated. ChrR of Pseudomonas putida is one such enzyme that has also been characterized as a chromate reductase; yet we propose that it is
Relationship between insulin sensitivity and gene expression in human skeletal muscle
Parikh HM, et al.
BMC endocrine disorders, 21, 32-32 (2021)
Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration
Veres-Szekely A, et al.
International Journal of Molecular Sciences, 23(4), 2119-2119 (2022)
-
What is the molecular weight of this diaphorase?
1 answer-
Helpful?
-
-
How to prepare stock solution for Diaphorase in order to use 2 units in reaction
1 answer-
For assay purposes, this product is reconstituted 1 mg/ml in cold 200 mM Tris-HCl buffer containing 294 mM Potassium Chloride, 0.54 mM Riboflavin 5'-monophosphate, and 0.025% (w/v) BSA, pH 7.5. The minimum activity of this enzyme is 30 u/mg protein with a minimum protein content of 50%. At the minimum activity and minimum protein content, 2 units of 1 mg/mL stock solution would have a volume of 133 uL. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d2197#product-documentationHelpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
