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D0942

Sigma-Aldrich

Anti-DHFR, C-terminal antibody produced in rabbit

1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-Dihydrofolate reductase

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About This Item

MDL number:
MFCD05665211
UNSPSC Code:
12352203
NACRES:
NA.56

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human, rat

concentration

1.0-1.5 mg/mL

technique(s)

immunoprecipitation (IP): 0.5-1.0 μg using 100-200 ng of purified DHFR
western blot (chemiluminescent): 0.5-1.0 μg/mL using 50-100 ng of purified recombinant DHFR, or 293T human embryonic kidney cell extract, or Jurkat human acute T Cell leukemia cell extract

UniProt accession no.

P00374

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DHFR(1719)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)

Specificity

DHFR and DHFR fusion proteins. The epitope resides within amino acids 171-185 of mouse DHFR.

Immunogen

amino acids 171-185 of mouse DHFR, conjugated to KLH via an N-terminal added cysteine residue.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Scientific reports, 9(1), 9987-9987 (2019-07-12)
Mitochondrial complex I (CI) is the largest multi-subunit oxidative phosphorylation (OXPHOS) protein complex. Recent availability of a high-resolution human CI structure, and from two non-human mammals, enabled predicting the impact of mutations on interactions involving each of the 44 CI
Neda Habibi Arejan et al.
Frontiers in microbiology, 13, 1085918-1085918 (2023-01-31)
Mycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but
Lauren A Howell et al.
Scientific reports, 9(1), 12418-12418 (2019-08-29)
The 26S proteasome conducts the majority of regulated protein catabolism in eukaryotes. At the heart of the proteasome is the barrel-shaped 20S core particle (CP), which contains two β-rings sandwiched between two α-rings. Whereas canonical CPs contain α-rings with seven

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