CE0050
CelLytic™ MEM Protein Extraction Kit
Sinónimos:
Membrane protein extraction kit
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Comprobar disponibilidad del carrito | US$ 537,00 |
Acerca de este artículo
NACRES:
NA.56
UNSPSC Code:
41116134
US$ 537,00
PRECIOS SIN IMPUESTOS NACIONALES
Comprobar disponibilidad del carrito
Servicio técnico
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Permítanos ayudarletechnique(s)
protein extraction: suitable
Quality Segment
input
mammalian sample(s)
shipped in
wet ice
storage temp.
−20°C
General description
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.
CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Application
CelLytic™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.
Sufficient reagents supplied for 80 tests.
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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Este artículo | |||
|---|---|---|---|
| description - | description Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. | description - | description For plant leaves |
| technique(s) protein extraction: suitable | technique(s) - | technique(s) - | technique(s) ChIP: suitable |
| storage temp. −20°C | storage temp. room temp | storage temp. −20°C | storage temp. 2-8°C |
| input mammalian sample(s) | input mammalian sample(s) | input bacteria and yeast sample(s) | input plant, fungi and insect sample(s) |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in wet ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Solo componentes del kit
Referencia del producto
Descripción
- Lysis and Separation Buffer 50 mL
- Wash Buffer for CelLytic MEM 50 mL
- Sodium Chloride, 4M Solution 1.5 mL
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS & Precios
- Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL
Clase de almacenamiento
10 - Combustible liquids
Punto de inflamación (°F)
188.6 °F - closed cup
Punto de inflamación (°C)
87 °C - closed cup
¿Qué está pasando?
WGK 2
Palabra de advertencia
Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2
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