Derived from mononuclear cells from peripheral blood of a 51 year old normal male. Cells were subsequently transformed with EBV. Fibroblasts designated 149BR (Sigma Catalogue number. 90011807) have been isolated from the same individual . This has been confirmed by STR profiling at ECACC. T cells from the same sample have been used for cellular radiosensitivity studies and cell survival analysis after UV-irradiation.
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.
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