Established by EBV transformation of mononuclear cells from the peripheral blood of a 51 year old male. T cells from the sample have been used as normal control for DNA repair studies and mutation analysis.
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5%CO2; 37°C. Cells form floating aggregates.
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