293-PERV-PK-CIRCE

97051411, human kidney, Not specified

• UNSPSC Code: 41106514

Propiedades

• product name: 293-PERV-PK-CIRCE, 97051411
• biological source: human kidney
• growth mode: Adherent aggregates
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human embryo kidney, infected with endogenous retrovirus of pigs

Cell Line Description

293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.

Application

Pig retrovirus studies, infectivity assays

Culture Medium

DMEM + 2mM Glutamine + 10% HI-FBS

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO₂ 3

Other Notes

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