Clone M-3

87081806, mouse skin, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: Clone M-3, 87081806
• biological source: mouse skin
• growth mode: Adherent
• karyotype: Hypertetraploid
• morphology: Epithelial
• products: Melanin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse (CxDBA)F1 melanoma

Cell Line Description

Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.

Application

Cloning studies

Culture Medium

Ham′s F10 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.