A9

85011426, mouse adipose tissue, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• product name: A9, 85011426
• biological source: mouse adipose tissue
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse C3H/An areolar and adipose tissue

Cell Line Description

Derivative of L929 selected in azaguanine (3μg/ml). Partially resistant to azaguanine. A9 may be heterozygous for the ability to synthesise active inosinic acid phosphorylase.

Application

Metabolic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-5x10,000/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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