Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 25 U/ml Interleukin-6 (IL-6) + 5-10% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA maybe required to dislodge them from the plastic.
Subculture Routine
Seed cultures between 1-3 x 105 cells/ml; Saturation density 8-10 x 105 5% CO2; 37 °C.
Other Notes
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