JB1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• envase: tube of 5 μg 01091808-DNA-5UG; pkg of vial of cells 01091808-1VL
• ImmunoDon: BALB/c B27 female transgenic mouse
• Mieloma: PS-NS1/Ag 4.1
• Reactividad: Anti- aggregated HFE protein, Human
• técnicas: cell culture | mammalian: suitable
• isotipo: IgG3
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Anti-recombinant HFE Protein hybridoma

Descripción línea celular

To produce this hybridoma mice transgenic HLA-B27 and human β-2 microglobulin were immunized with a preparation of HFE made in recombinant bacteria, refolded with human β-2 microglobulin. Characterisation beyond data in B.J. Haematol 103, 931, 1998 shows that JB1 reacts predominantly with aggregated denatured HFE produced in bacteria, but does not bind folded recombinant HFE produced in CHO cells. In comparison with polyclonal sera raised to the C terminus or the α 3 region of HFE JB1 stains frozen sections of the tissues well, but reacts very weakly with cells transfected with the HFE gene in vitro (usually only visible by confocal microscopy). JB1 does not stain paraffin fixed sections and will not work in western blots.

Inmunógeno

Bacterial recombinant HFE protein

Medio de cultivo

RPMI 1640, 10% FBS, 1% HAT

Rutina de subcultivo

Maintain cells at 3-5x100,000 cells/ml which is usually a split ratio of 1:2. Saturation density os 5 x 100,000 to 1 x 1000,000.

Otras notas

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