C7988
Anti-Cre antibody, Mouse monoclonal
clone 7-23, purified from hybridoma cell culture
Sinónimos:
Mouse Anti-Recombinase cre
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About This Item
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Productos recomendados
origen biológico
mouse
conjugado
unconjugated
forma del anticuerpo
purified from hybridoma cell culture
tipo de anticuerpo
primary antibodies
clon
7-23, monoclonal
Formulario
buffered aqueous solution
mol peso
antigen ~38 kDa
concentración
~2 mg/mL
técnicas
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using recombinant Cre recombinase
isotipo
IgG1
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Categorías relacionadas
Descripción general
Monoclonal Anti-Cre (mouse IgG1 isotype) is derived from the 7-23 hybridoma produced by the fusion of mouse myeloma cells (X63-Ag8.653) and splenocytes from BALB/c mice immunized with Cre protein. Cre recombinase is a member of the integrase family of proteins that are site-specific recombinases.The protein is expressed in P1 bacteriophage.
Aplicación
Anti-Cre antibody ,Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunohistochemistry
- immunocytochemistry
- flow cytometry
- immunostaining
Acciones bioquímicas o fisiológicas
Cre recombinase enables DNA recombination between two recognition sites called loxP. Each one of these sites (34 bp long) contain two 13 bp palindromic flanking sequences and in between them is a core spacer sequence 8 bp long. The recombination event takes place within the spacer area of the two loxP sites. Cre/loxP system manipulates genes in mammalian systems by making deletions, gene replacements, insertions (knockin), conditional gene targeting (knockout), and point mutations. Monoclonal antibodies to the Cre protein are an important tool for the identification of Cre recombinase in different mammalian systems.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Almacenamiento y estabilidad
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution should be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Directional resolution of synthetic holliday structures by the Cre recombinase
Lee L and Sadowski PD
Test, 276(33), 31092-31098 (2001)
Dopamine receptor D2, but not D1, mediates descending dopaminergic pathway-produced analgesic effect in a trigeminal neuropathic pain mouse model
Liu S, et al.
Pain, 160(2), 334-334 (2019)
Fernando H Biase et al.
iScience, 7, 16-29 (2018-09-30)
We developed the Rainbow-seq technology to trace cell division history and reveal single-cell transcriptomes. With distinct fluorescent protein genes as lineage markers, Rainbow-seq enables each single-cell RNA sequencing (RNA-seq) experiment to simultaneously decode the lineage marker genes and read single-cell
Francesco Trovato et al.
Nature communications, 11(1), 6194-6194 (2020-12-05)
Genetic mosaicism, a condition in which an organ includes cells with different genotypes, is frequently present in monogenic diseases of the central nervous system caused by the random inactivation of the X-chromosome, in the case of X-linked pathologies, or by
Susana Martin-Ortigosa et al.
Methods in molecular biology (Clifton, N.J.), 1642, 169-180 (2017-08-18)
We describe a non-DNA-based system for delivering Cre recombinase protein into maize tissue using gold-plated mesoporous silica nanoparticle (Au-MSN). Cre protein is first loaded into the pores of Au-MSNs and then delivered using the biolistic method to immature embryos of
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