This product contains EDTA as the major active component, which acts as a chelating agent for divalent cations to loosen cell–cell contacts but does not break down the extracellular matrix as trypsin does. Empirical testing is needed to determine if this approach is more effective. It is highly recommended to review the literature to determine if an EDTA-only dissociation method has been successfully used with the particular primary microglia preparation protocol.
C5789
Cell Dissociation Solution Non-enzymatic 1x
Prepared in Hanks′ Balanced Salt Solution without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture
Sinónimos:
Cell Dissociation Reagent
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
100 mL | Comprobar disponibilidad del carrito | US$ 66,00 |
Acerca de este artículo
Concentration:
1 ×
US$ 66,00
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Comprobar disponibilidad del carrito
Servicio técnico
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Permítanos ayudarleQuality Segment
sterility
sterile-filtered
product line
BioReagent
form
solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
pH
6.5-7.5
shipped in
wet ice
storage temp.
room temp
General description
Cell Dissociation Solution Non-enzymatic buffer systems are alternative to the canonical trypsin based buffers for dissociation of adherent mesenchymal stem cell (MSC) monolayers.
Application
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.
Cell Dissociation Solution Non-enzymatic 1x has been used in the dissociation of murine macrophage (RAW2647) cells, A549 pulmonary cells, islets cells and 2D cell and 3D mammospheres.
1 of 1
Este artículo | |||
|---|---|---|---|
| description Prepared in Hanks′ Balanced Salt Solution without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture | description 1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red | description Chemically defined enzyme-free organoid dissociation solution. | description sterile-filtered, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | stem cell: suitable | technique(s) single cell analysis: suitable, cell culture | mammalian: suitable |
| form solution | form solution | form liquid | form solution |
| concentration 1 × | concentration 1 × | concentration - | concentration 1 × |
| storage temp. room temp | storage temp. −20°C | storage temp. - | storage temp. −20°C |
| sterility sterile-filtered | sterility sterile; sterile-filtered | sterility - | sterility sterile-filtered |
| shipped in wet ice | shipped in dry ice | shipped in - | shipped in dry ice |
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Clase de almacenamiento
12 - Non Combustible Liquids
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WGK 1
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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Hi, I am working with primary microglia and it very tricky to dissociate. I usually dissociate them with 0.5% Trypsin for 7 min at 37˚C and still hard to collect them. May I know whether this reagent would be a better alternative? Thank you!
1 answer-
Helpful?
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What is the concentration of EDTA in Cat#C5789? Thanks
1 answer-
The concentration of EDTA in this formulation is proprietary. Additional components include Sodium Citrate, Nitrilotriacetic Acid, and Glycerol in Hank's Balanced Salt solution. Should the concentration of EDTA be required, product E8008, EDTA 0.2% in DPBS, may be of interest. Both items are tested for cell dissociation. Please see the link below to review this product option:
https://www.sigmaaldrich.com/US/en/product/sigma/e8008Helpful?
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