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B4882

Bromelain from pineapple stem

≥3 units/mg protein

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Talla/SKUDisponibilidadPrecio
10 g

Disponible para enviar HOYdesdeCarlos Spegazzini

US$ 111,00
25 g
Comprobar disponibilidad del carrito
US$ 204,00
100 g
Comprobar disponibilidad del carrito
US$ 643,00
500 g

Disponible para enviar HOYdesdeCarlos Spegazzini

US$ 2.220,00
1 kg
Comprobar disponibilidad del carrito
US$ 4.000,00

Acerca de este artículo

Número CAS:
37189-34-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-387-5
MDL number:
MFCD00081423
Número CE:
3.4.22.32 (BRENDA, IUBMB)
Specific activity:
≥3 units/mg protein

US$ 111,00

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form

lyophilized powder

Quality Segment

specific activity

≥3 units/mg protein

composition

Protein, ≥30% biuret

storage temp.

−20°C

Application

Bromelain from pineapple stem has been used:
  • to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants
  • as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay
  • to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences

Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Preparation Note

The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.

Other Notes

One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).

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Este artículo
B51449385452549
Bromelain from pineapple stem ≥3 units/mg protein

Sigma-Aldrich

B4882

Bromelain from pineapple stem

Bromelain from pineapple stem ≥4 units/mg protein, (chromatography purified)

Sigma-Aldrich

B5144

Bromelain from pineapple stem

Pectin from apple

Sigma-Aldrich

93854

Pectin from apple

Melamine analytical standard

Supelco

52549

Melamine

description

≥3 units/mg protein

description

≥4 units/mg protein, (chromatography purified)

description

-

description

analytical standard

specific activity

≥3 units/mg protein

specific activity

(chromatography purified), ≥4 units/mg protein

specific activity

-

specific activity

-

form

lyophilized powder

form

lyophilized powder

form

powder

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

composition

Protein, ≥30% biuret

composition

Protein, 30-50% biuret

composition

-

composition

-

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Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de advertencia

Danger

Códigos de peligro

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos diana

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

¿Qué está pasando?

WGK 3

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. We plan to use B4882 bromelain to hydrolyze the protein matrix of vitamin A beadlets during sample preparation before saponification. Could you provide its activity in GDU/g and confirm whether it complies with Ph. Eur. Bromelains R?

    1 answer

    1. The activity of this product is established using the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide. The Gelatin Digesting Unit activity has not been determined. Historical records indicate that 1.0 chromogenic unit is the equivalent of approximately 0.59 gelatin digestion units. This product is not tested according to, and does not meet, any compendial or agency standards, including the European Pharmacopoeia, Bromelain R.

      Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/282/040/b4882pis.pdf

      Helpful?

  2. I WILL USE BROMELAIN (B4882-10G ).IN A RESEARCH PROJECT. BROMELAIN IS COMPOSED OF MANY ENZYMES. THEY WERE ASKED WHAT the PROPERTIES AND CONTENT OF THE BROMELAIN YOU USE. I WOULD BE HAPPY IF YOU HELPED ME WITH

    1 answer

    1. The properties and content of this product have not been determined. The percentage of protein is lot specific. The Certificate of Analysis (CoA) provides information on the quality control release tests.

      Helpful?

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