B2059

Biotin Polyethyleneoxide Iodoacetamide

• Sinónimos: Biotin PEO Iodoacetamide
• Fórmula empírica (notación de Hill): C₁₈H₃₁IN₄O₅S
• Peso molecular: 542.43
• Número MDL: MFCD04974830
• Código UNSPSC: 12352119
• ID de la sustancia en PubChem: 24891627
• NACRES: NA.32

Propiedades

• descripción: Sulfhydryl specific, water soluble biotinylation reagent
• Formulario: powder
• solubilidad: H₂O: 10 mg/mL
• temp. de almacenamiento: 2-8°C
• cadena SMILES: ICC(=O)NCCOCCOCCNC(=O)CCCCC1SCC2NC(=O)NC12
• InChI: 1S/C18H31IN4O5S/c19-11-16(25)21-6-8-28-10-9-27-7-5-20-15(24)4-2-1-3-14-17-13(12-29-14)22-18(26)23-17/h13-14,17H,1-12H2,(H,20,24)(H,21,25)(H2,22,23,26)
• Clave InChI: LWGLKVINXNMNJX-UHFFFAOYSA-N

Descripción

Descripción general

Biotin polyethyleneoxide (PEO) iodoacetamide is a sulfhydryl (thiol) specific biotinylation reagent.[1]

Aplicación

• Incorporates a 12 atom hydrophilic spacer.
• Typically coupled to sulfhydryl groups at pH 7.5 - 8.5
• Especially useful for cysteine labeling in many proteomics type applications such as peptide mapping and mass spectrometry.

Biotin Polyethyleneoxide Iodoacetamide has been used as a thiol reactive reagent to demonstrate the presence of free thiols in capsid protein L1 of human papillomavirus 16.[2] It has been used for biotinylation of proteins for ELISA (enzyme-linked immunosorbent assay) competition and pull down experiments.[3][4]

Acciones bioquímicas o fisiológicas

Biotin polyethyleneoxide (PEO) iodoacetamide group reacts specifically with reduced thiols at pH 7.5 - 8.5. Peptides and small molecules containing thiol groups may also be labeled using this reagent. The biotin PEO iodoacetamide enables tagging of cysteine residues and conjugation to sulfhydryls in proteins making it useful in proteomics, peptide mapping, phosphopeptide analysis, and mass spectrometry.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H315,H319,H335
• Consejos de prudencia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves