Saltar al contenido
Merck

Documentos clave

Ver Toda la documentación

Saltar a

A7653

L-Alanine Dehydrogenase from Bacillus subtilis

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

Sinónimos:

L-Alanine: NAD+ oxidoreductase (deaminating)

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
A ustedes/SKUDisponibilidadPrecio
100 units
Comprobar disponibilidad del carrito
US$ 428,00

Acerca de este artículo

Número CAS:
9029-06-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-847-9
MDL number:
MFCD00131441
Número CE:
1.4.1.1 (BRENDA, IUBMB)
Specific activity:
~30 units/mg protein (Lowry)
Biological source:
Bacillus subtilis

US$ 428,00

PRECIOS SIN IMPUESTOS NACIONALES
Comprobar disponibilidad del carrito
​
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle

biological source

Bacillus subtilis

Quality Segment

200

form

buffered aqueous glycerol solution

specific activity

~30 units/mg protein (Lowry)

foreign activity

LDH ~1% (using pyruvate as substrate)

storage temp.

−20°C

Application

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection [1].

Biochem/physiol Actions

L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order.[1] Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.[2]

Physical form

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

Other Notes

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C.

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo
A7189L5135A9378
L-Alanine Dehydrogenase from Bacillus subtilis buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

Sigma-Aldrich

A7653

L-Alanine Dehydrogenase from Bacillus subtilis

L-Alanine Dehydrogenase from Bacillus subtilis ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

Sigma-Aldrich

A7189

L-Alanine Dehydrogenase from Bacillus subtilis

L-Leucine Dehydrogenase from Bacillus cereus lyophilized powder, ≥60 units/mg protein

Sigma-Aldrich

L5135

L-Leucine Dehydrogenase from Bacillus cereus

L-Amino Acid Oxidase from Crotalus adamanteus Type IV, ≥4.0 units/mg protein, aqueous suspension

Sigma-Aldrich

A9378

L-Amino Acid Oxidase from Crotalus adamanteus

biological source

Bacillus subtilis

biological source

Bacillus subtilis

biological source

-

biological source

-

specific activity

~30 units/mg protein (Lowry)

specific activity

≥20 units/mg protein (Lowry)

specific activity

≥60 units/mg protein

specific activity

≥4.0 units/mg protein

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

lyophilized powder

form

aqueous suspension

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

foreign activity

LDH ~1% (using pyruvate as substrate)

foreign activity

-

foreign activity

-

foreign activity

-

Still not finding the right product?

Explore all of our products under L-Alanine Dehydrogenase from Bacillus subtilis

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Questions

Reviews

No rating value

Active Filters